Bioinspired artificial water channels aim to combine the high permeability and selectivity of biological aquaporin (AQP) water channels with chemical stability. We characterized a new architecture of artificial water channels, peptide-appended pillararenes (PAPs). The average single-channel osmotic water permeability for PAPs is 1.0(± 0.3)×10−14 cm3/s or 3.5(± 1.0)×108 water molecules/s, which is in the range of AQPs (3.4~40.3×108 water molecules/s) and their current synthetic analogs, carbon nanotubes (CNTs, 9.0×108 water molecules/s). This permeability is an order of magnitude higher than first-generation artificial water channels (20 to ~107 water molecules/s). Furthermore, within lipid bilayers, PAP channels can self-assemble into two-dimensional arrays. Importantly for permeable membrane design, the pore density of PAP channel arrays (~2.6×105 pores/μm2) is two orders of magnitude higher than CNT membranes (0.1~2.5×103 pores/μm2). PAP channels thus combine the advantages of biological channels and CNTs and improve upon them through their relatively simple synthesis, chemical stability and propensity to form arrays.