%0 Journal Article %J Nano Letters %D 2018 %T Optical voltage sensing using DNA origami %A Hemmig, Elisa Alina %A Fitzgerald, Clare %A Maffeo, Christopher %A Hecker, Lisa %A Ochmann, Sarah Elisabeth %A Aleksei Aksimentiev %A Tinnefeld, Philip %A Keyser, Ulrich F %X

We explore the potential of DNA nanotechnology for developing novel optical voltage sensing nano-devices that convert a local change of electric potential into optical signals. As a proof-of-concept of the sensing mechanism, we assembled voltage responsive DNA origami structures labelled with a single pair of FRET dyes. The DNA structures were reversibly immobilised on a nanocapillary tip and underwent controlled structural changes upon application of an electric field. The applied field was monitored through a change in FRET efficiency. By exchanging the position of a single dye, we could tune the voltage sensitivity of our DNA origami structure, demonstrating the flexibility and versatility of our approach. The experimental studies were complemented by coarse-grained simulations that characterised voltage-dependent elastic deformation of the DNA nanostructures and the associated change in the distance between the FRET pair. Our work opens a novel pathway for determining the mechanical properties of DNA origami structures, and highlights potential applications of dynamic DNA nanostructures as voltage sensors.

%B Nano Letters %V 18 %P 1962-1971 %G eng %U https://doi.org/10.1021/acs.nanolett.7b05354 %R 10.1021/acs.nanolett.7b05354 %0 Journal Article %J Methods Mol Biol %D 2012 %T Optimization of the molecular dynamics method for simulations of DNA and ion transport through biological nanopores %A David B Wells %A Bhattacharya, Swati %A Carr, Rogan %A Christopher Maffeo %A Anthony H. Ho %A Jeffrey Comer %A Aleksei Aksimentiev %K Biological Transport %K DNA %K Electric Conductivity %K Electroosmosis %K Hemolysin Proteins %K Ions %K Lipid Bilayers %K Molecular Dynamics Simulation %K Nanopores %K Porins %K Solvents %K Thermodynamics %X

Molecular dynamics (MD) simulations have become a standard method for the rational design and interpretation of experimental studies of DNA translocation through nanopores. The MD method, however, offers a multitude of algorithms, parameters, and other protocol choices that can affect the accuracy of the resulting data as well as computational efficiency. In this chapter, we examine the most popular choices offered by the MD method, seeking an optimal set of parameters that enable the most computationally efficient and accurate simulations of DNA and ion transport through biological nanopores. In particular, we examine the influence of short-range cutoff, integration timestep and force field parameters on the temperature and concentration dependence of bulk ion conductivity, ion pairing, ion solvation energy, DNA structure, DNA-ion interactions, and the ionic current through a nanopore.

%B Methods Mol Biol %V 870 %P 165-86 %8 2012 %G eng %R 10.1007/978-1-61779-773-6_10 %0 Journal Article %J Proc Natl Acad Sci U S A %D 2005 %T Orientation discrimination of single-stranded DNA inside the alpha-hemolysin membrane channel %A Mathé, Jérôme %A Aleksei Aksimentiev %A Nelson, David R %A Klaus Schulten %A Meller, Amit %K Bacterial Toxins %K Base Sequence %K Biophysical Phenomena %K Biophysics %K DNA, Single-Stranded %K Hemolysin Proteins %K Ion Channels %K Models, Molecular %K Motion %K Nucleic Acid Conformation %K Poly A %K Thermodynamics %X

We characterize the voltage-driven motion and the free motion of single-stranded DNA (ssDNA) molecules captured inside the approximately 1.5-nm alpha-hemolysin pore, and show that the DNA-channel interactions depend strongly on the orientation of the ssDNA molecules with respect to the pore. Remarkably, the voltage-free diffusion of the 3'-threaded DNA (in the trans to cis direction) is two times slower than the corresponding 5'-threaded DNA having the same poly(dA) sequence. Moreover, the ion currents flowing through the blocked pore with either a 3'-threaded DNA or 5' DNA differ by approximately 30%. All-atom molecular dynamics simulations of our system reveal a microscopic mechanism for the asymmetric behavior. In a confining pore, the ssDNA straightens and its bases tilt toward the 5' end, assuming an asymmetric conformation. As a result, the bases of a 5'-threaded DNA experience larger effective friction and forced reorientation that favors co-passing of ions. Our results imply that the translocation process through a narrow pore is more complicated than previously believed and involves base tilting and stretching of ssDNA molecules inside the confining pore.

%B Proc Natl Acad Sci U S A %V 102 %P 12377-82 %8 2005 Aug 30 %G eng %N 35 %R 10.1073/pnas.0502947102