%0 Journal Article %J ACS Nano %D 2016 %T Large-Conductance Transmembrane Porin Made from DNA Origami %A Kerstin Göpfrich* %A Chen-Yu Li* %A Maria Ricci %A Satya Prathyusha Bhamidimarri %A Jejoong Yoo %A Bertalan Gyenes %A Alexander Ohmann %A Mathias Winterhalter %A Aleksei Aksimentiev %A Ulrich F Keyser %X

DNA nanotechnology allows for the creation of three-dimensional structures at nanometer scale. Here, we use DNA to build the largest synthetic pore in a lipid membrane to date, approaching the dimensions of the nuclear pore complex and increasing the pore-area and the conductance tenfold compared to previous man-made channels. In our design, nineteen cholesterol-tags anchor a megadalton funnel-shaped DNA origami porin in a lipid bilayer membrane (see equilibration trajectory, require login to nanoHUB). Confocal imaging and ionic current recordings reveal spontaneous insertion of the DNA porin into the lipid membrane, creating a transmembrane pore of tens of nanosiemens conductance (see ionic current trajectory). All-atom molecular dynamics simulations characterize the conductance mechanism at the atomic level and independently confirm the DNA porins' large ionic conductance.

%B ACS Nano %V 10 %P 8207--8214 %8 08/2016 %G eng %N 9 %R 10.1021/acsnano.6b03759